YES, that stand-off in your picture CAN cause a big problem. Any stand-off NOT where there is a mounting hole in the mobo can cause a short circuit to Ground from a trace line on the bottom of the mobo. You cannot have that in your system!
That said, obviously this can not impact your system in your out-of-case test setup. Just to be sure that was done right, this is the start of "breadboard" testing. The mobo is on a non-conductive surface. You MUST have installed on that board:
CPU chip and cooler system
Cooling device (fan?) for the CPU installed AND plugged into the CPU_FAN header
1 stick of RAM in the correct slot for single stick operation - manual p. 4, slot DIMMA2
Video card in PCIe slot and connected to monitor
PSU connected to board (and video card if needed)
You use a small screwdriver to short out briefly the 2 pins on the mobo's Front Panel header (manual, p. 34, JFP1, pins 6 and 8) labelled Power Switch to trigger a start-up.
Without those minimal items installed the board cannot start. If it works, it will POST properly but end with an error message that it had no boot device (the SSD unit that is NOT installed).
If that does not start up and complete POST, there is a problem with the mobo OR your PSU. That misplaced stand-off MIGHT have caused damage to the mobo, OR to the PSU - even the new one if you used that with the original system in the case. Can you borrow a PSU from somewhere and try that in the breadboard set-up?
If you can get it working outside the case, post back here for how to re-locate that misplaced stand-off.
I tried this minimal configuration buy sadly no luck on display. Unfortunately, no one buys desktops anymore, all of my friends have laptops. So can't test. I ran this experiment though from reddit:
Any damage to that part of the motherboard?
No, nothing at all. Physically, it's 100% fine
Start w/ the CPU installed only.
Clear CMOS before attempting this.
Connect the PSU up and boot the system.
Ideally you should see the RAM lights come on and stay on
Yes, RAM LEDs stay on. It even beeps complaining about no RAM.
reboot 3 or 4 times more and make sure you get the same consistent behavior
Yes, it is consistent
Install the RAM next and the GPU light should come on and stay on
I have two RAMs and slots are marked as A1,A2, B1,B2. Manual says:
I used single RAM, so I put in A2.
It never complains about GPU. GPU LED never comes on. Guess what remains on? BOOT LED. Rebooted 4 times and all times same behavior
So, after all of the above, since BOOT LED was coming on, next I installed SSD (M.2) then and booted it up. No LED lights turned on. I rebooted 4 times to conform this. All fine.
Now I put graphics card this time, No LED lights turn on. It's running but no display on monitor. Monitor says no signal detected.
I put in the 2nd RAM and BOOT LED comes up. I rebooted 4 times to confirm this.
So I go back to just single RAM but BOOT LED remains on. Then I removed graphics card, still BOOT LED on, Then I shut off the machine for few minutes, turn the PSU off using the button at the back. 5 min later I turn it on and still BOOT LED comes up. I removed SSD too. Still BOOT LED remains on.
Honestly I can't think of anymore ideas and experiments now. I might just stash the entire desktop in one corner and focus in saving money to get a new one, Even a new PSU