Yes. ATA natively transfers data in 16 bit chunks, yet the PCI bus it is normally attached to is 32 bit, so half the bandwidth goes to waste for each transfer. When 32bit transfers are enabled, the controller waits for 2 packets and groups them together before sending them on their way. Most motherboards have this feature enabled by default and don't even give you the option of turning it off, since there really is no reason to ever turn it off. Don't expect any preceptive speed increases, but again there is no reason not to enable it.