Yes. that is exactly why people love Norton Ghost so much. Great for 1) moving to a bigger HDD in the same system; cloning your setup to identical or nearly identical systems (assuming you have bought the necessary licenses of course.)
Two things though. Make sure that the jumpers, cable position on the new drive you ghosted to are set to be primary, not slave, when you want to boot off of it.
Second, you have to set the disk up as a primary bootable disk in Fdisk. I BELIEVE you can do this after the fact, without destroying data. But it would be wise to do it before the fact the first time, since I haven't done it in a while.
If you want to clone your setup to a signficantly different bunch of hardware, it is more complicated. If the hardware is radically different, it would be wise to simply reinstall everything, and ghost or otherwise copy over the data files only.
If the systems aren't that radically different, if SHOULD/MIGHT work to try this:
1) ghost to the new HDD, after it has been FDISHED as having a boot partition.
2) switch cables to boot from the new HDD on the old computer;
3) go into My computer (right click) device manager and delete all the hardware which is different on the new computer. Don't delete the HDD stuff, it's booting ok on that. Don't delete any more of the basic system stuff than is necessary (this is the edge of my knowledge, e.g. if you are changing basic mobo chipsets. I'd got light here and hope for the best. If it didn't work I'd put the drive back in the old computer, boot again with stuff disabled, let it find stuff, and then delete more basic stuff.) Actually I think the right answer I saw someplace around here was to delete everything EXCEPT X, when it a major system change. What's X???
4) fire the new HDD up in the new system, with your OS CD at the ready, and any driver CD's, floppy download updates, for your new hardware. Let it find all sorts of hardware. For the first pass, have as little hardware in/attached as possible. Only video card perhaps. Then add sound card the next boot, etc. Finally plug in your printer.
5) If you are lucky, plug and play will pull it through for you.