only on win98se you need drivers, and i'm assuming anything before that that supports USB... you can download them from the website. these are great, i use them exclusively now, no more floppy pains! the only hassle is reaching to the back of some of the computers! They have to make front USB ports standard on all machines! I don't know how i lived without mine!
By the way i got the diskonkey 32mb @ $40 after a dell deal a few months ago, this is a great price IMHO! (I'd actually go for the 64MB! You can't go wrong with more!). I believe the diskonkey brand v2 can be used as a bootable usb drive!