General Zod
Green Goblin
Doctor Octopus
Deathstrike (just because she kicked Wolverine's ass all over the place)
Optimus Prime; after the Decepticons trashed Autobot city and killed several of them, he trashed the entire Decepticon army BY HIMSELF. After getting a mortal wound, he still clocked Megatron hard enough to do severe damage
John Preston: Equilbrium
Blade and Deacon Frost from Blade
Agent Smith from The Matrix
Wesley from The Pricess Bride
The Stay Puff Marshmellow Man (and the ghost librarian, while I'm at it)
The Thing from The Thing
The Joker (Specifically in Batman Mask of the Phantasm and Return of the Joker)
The worms in Tremors
Paul Muad'b from Dune