No, the RAM will only run underclocked. PC-100 is only a standard that certifiates that this row of RAM can support 100MHz. So, PC-100 will work in a PC-66 slot, but will only work at 66MHz. You can also put PC-133 RAM in a PC-66 slot, it'll do the same thing. But, why purchase something to make it run at half the speed it could go...