You could try booting into DOS.... If you are unfamiliar with it it can be a little confusing.
Write down the path that the file is in. If you have any folder names greater than 8 characters, stop after the 6th character and add ~1. This is where the file is on a DOS filesystem.
Now boot into DOS. Some would tell you that Restart in MS-DOS mode isn't a good way to do it, but it is the simplest way to get you there.
((If you want to do it the best way, reboot and press F8 at the memory test screen. It will either take you directly to the comand prompt [the old fashioned "C:\WINDOWS\>"] or take you to the boot menu that gives you the option of booting to the comand prompt.))
Once you have a comand prompt, if the file is not on C drive, type the letter of the drive + : -- ie. if it is on Z drive type Z:
Then type cd ((the path you wrote out before))
Then type dir ((filename)) to verify if you have the right folder. Careful, filenames follow the same naming procedure as folders... and you have to add the extension. ((Also known as the file type - .exe .jpg .mp3 .mpg etc.))
You should recieve a few lines that lists the file name, its size in Bytes, last modified date, etc. If you do not recieve this, you have the wrong folder.
If you have gotten this far, congatulations. Now all you have to do is type del ((filename))
After that, reboot. From DOS you can press the reset button or the power button. Or, you could try and type exit it works most of the time.
That should fix your problem.
DISCLAIMER!!!! This may cause you more problems than it is worth. I do not know what the file is or what is trying to access it or why. Delete this file at your own risk.
Good luck to you!